Radiation Hybrid (RH) Map

Two ovine radiation hybrid panels have been constructed:

Currently 1000 EST and microsatellite markers are being mapped to the US panel; and 61 markers have been mapped to a 23 Mb region from the INRA panel.

The consortia plan to map representative BAC end sequences from the virtual genome and 60K SNP chip over both resources. This will provide a dense high quality RH map independently linking the BAC-comparative genome contigs (BAC-CGCs) together as well as confirming their order and orientation.

RH mapping gel
Figure 1 RH agarose gel showing presence or absence of bands for each clone using a specific PCR probe